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Barcelona said Eto'o may have to undergo surgery and was expected to return to training in eight weeks.
Eto'o spent five months on the sidelines last season with a torn cruciate ligament in his left knee.
The Cameroon striker's absence will at least solve coach Frank Rijkaard's selection headache for a while.
Rijkaard has the task of deciding between a quartet of top forwards - Eto'o, Ronaldinho, Lionel Messi and recent signing Thierry Henry.
In Barcelona's opening game of the season against Racing Santander last Sunday, Rijkaard left Henry on the bench until the second half when he replaced Messi. The game ended scoreless.
Barcelona hosts Athletic Bilbao on Sunday. Pravda.ru